Tetraethylene Glycol-Derived Spacer for
Oligonucleotide Synthesis
3,6,9-Trioxaundecane-1,11-diisocyanate was synthesised from tetraethylene
glycol in 5 steps and 48 % overall yield. The spacer was monofunctionalised
with a fully protected adenosyl-3'-O-succinate derivative and linked to
aminomethyl polystyrene (50 % crosslinked with divinylbenzene) affording
a solid support suitable for oligoribonucleotide synthesis (loading: ~20
µmol/g). The HPLC analysis of a crude oligoribonucleotide synthesis
and the isolated yield of purified oligomer show that this spacer compares
well to the commercially used hexamethylene diamine.
